If you defrosted the bottle of E3Live in your refrigerator (and left it a few days) or in a cool bowl of water and it hasn't been thawed for more than a few hours, it will be fine. It is best to place your algae into individual servings and freeze immediately to preserve its nutritional integrity.
QUICK AND EASY ICE CUBE METHOD: individual servings (favorite method)
1. Pour and Measure: Pour thawed E3Live or BrainON into an ice cube tray, or individual serving cups, trays, silicone molds, etc ensuring each cube equals one serving.
2. Freeze: Place tray or cups into the freezer until the cubes are solid.
3. Store: Keep the cubes in an airtight container to maintain freshness.
NOTE:
If the E3Live has thawed out in a very hot room, has been out for many hours, has thawed outside in the sun, or some similar undesirable condition - check it for color and smell. If it spoils, it will turn a muddy brown color and have a rancid smell that will tell you that it has spoiled. If this happens - you can always mix it with water for an awesome plant fertilizer!